Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743444 | Analytica Chimica Acta | 2005 | 6 Pages |
Abstract
A new assay of nucleic acids at nanogram level was established based on the enhanced resonance light scattering (RLS) signals of two zwitterionics cocamidopropyl hydroxysultaine (HSB) and lauryl betaine (BS-12). Under optimum conditions, the weak RLS signal of HSB is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids in the range of 0.02-7.3 mg lâ1 for calf thymus DNA and 0.01-8.6 mg lâ1 for fish sperm DNA. The detection limits were 1.5 ng mlâ1 for calf thymus DNA and 1.9 ng mlâ1 for fish sperm DNA. Plasmid DNA extracted from K-12-HB101 colt was determined with satisfactory results.
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Authors
Zhanguang Chen, Weifeng Ding, Fenglian Ren, Jinbin Liu, Yizeng Liang,