Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743455 | Analytica Chimica Acta | 2005 | 6 Pages |
Abstract
A new spectrofluorimetric method was developed for the determination of trace amount of nicotinamide adenine dinucleotide phosphate (NADP). Using europium (Eu3+)-tetracycline (TC) complex as a fluorescent probe, in the buffer solution of pH 7.60. NADP can remarkably enhance the fluorescence intensity of the Eu3+-TC complex at λ = 612 nm and the enhanced fluorescence intensity of Eu3+ ion is in proportion to the concentration of NADP. Optimum conditions for the determination of NADP were also investigated. The dynamic range for the determination of NADP is 4.4 Ã 10â7 to 2.2 Ã 10â6 mol lâ1 with detection limit of 6.9 Ã 10â8 mol lâ1. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of NADP in synthetic water samples and in serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the Eu3+-TC system and the Eu3+-TC-NADP system have been also discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qian Peng, Faju Hou, Xiaoxia Ge, Chongqiu Jiang, Shubo Gong,