Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743464 | Analytica Chimica Acta | 2005 | 8 Pages |
Abstract
A novel and simple capillary zone electrophoresis method was developed and validated for the simultaneous determination of rosiglitazone and metformin in pharmaceutical preparations. The optimum separation for these analytes was achieved in <9 min at 25 °C with a fused-silica capillary column (80.5 cm Ã 75 μm i.d., effective length 72.0 cm) and a running buffer containing 25 mM acetate buffer at pH 4.0. The samples were injected hydrodynamically for 3 s at 50 mbar and the applied voltage was +25.0 kV. Detection wavelength was set at 203 nm. Verapamil was used as internal standard. The method was suitably validated with respect to specificity, linearity, limit of detection and quantitation, accuracy, precision, and robustness. The limits of detection and quantitation were 0.5 and 1.0 μg mLâ1 for rosiglitazone and metformin, respectively. The method developed was successfully applied to the simultaneous determination of rosiglitazone and metformin in pharmaceutical preparations. The results were compared to a liquid chromatographic method reported in the literature and no significant difference was found statistically.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ceren Yardımcı, Nuran Ãzaltın,