Optimization of the chromatographic conditions for the determination of hormones by gas chromatography with mass spectrometry detection

A two-factor Doehlert design was subsequently performed to fit a second-order model and jointly optimize the resolution and the peak width through a global desirability function. Thus, the final temperatures of the first and the second ramp are 260 and 300 °C, respectively. The method developed has been validated according to the European Decision 2002/657/EC, including the estimation of the capability of detection (CCβ, X0 = 0, for banned substances) with evaluation of the probability of false positive, α, and of false negative, β.