Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743602 | Analytica Chimica Acta | 2005 | 12 Pages |
Abstract
Bio- and chemiluminescence imaging techniques combine the high sensitivity of bio- and chemiluminescence detection with the ability of current light imaging devices to localize and quantify light emission down to the single-photon level. These techniques have been successfully exploited for the development of sensitive analytical methods relying on the evaluation of the spatial distribution of the light emitted from a target sample. In this paper, we report on recent applications of bio- and chemiluminescence imaging for in vitro and in vivo assays, including: quantitative assays performed in various analytical formats, such as microtiter plates, microarrays and miniaturized analytical devices, used in the pharmaceutical, clinical, diagnostic and environmental fields; luminescence imaging microscopy based on enzymatic, immunohistochemical and in situ hybridization reactions for the localization of metabolites, enzymes, antigens and gene sequences in cells and tissues; whole-body luminescence imaging in live animals for evaluating biological and pathological processes and for pharmacological studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Aldo Roda, Massimo Guardigli, Patrizia Pasini, Mara Mirasoli, Elisa Michelini, Monica Musiani,