| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9743608 | Analytica Chimica Acta | 2005 | 5 Pages |
Abstract
Hot electrons can be injected from conductor/insulator/electrolyte (C/I/E) junctions into an aqueous electrolyte solution. Injected hot electrons induce electrogenerated chemiluminescence (ECL) of various luminophores in fully aqueous solutions. Such ECL gives the basis of electrochemiluminoimmunoassays and DNA-probe assays where different luminophores can be used as electrochemiluminescent labels. This work shows that SYBR® Green I is suitable as an ECL label in detection methods based on C/I/E tunnel-emission electrodes such as oxide-coated aluminium, magnesium and silicon electrodes.
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Authors
Pirita Laakso, Hannamari Anttila, Veli Kairisto, Jarkko Eskola, Sakari Kulmala, Timo Ala-Kleme,