Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743721 | Analytica Chimica Acta | 2005 | 6 Pages |
Abstract
Pressurized liquid extraction (PLE) along with normal phase liquid chromatography (NPLC) is evaluated as a new approach for the determination of β-carotene and Vitamin E isomers in residue oil obtained from palm pressed fiber (PPF). The relative extraction recoveries obtained by optimized PLE were found to be equivalent (â¼100%) to conventional Soxhlet extraction. Optimized PLE was obtained (5 g of sample, 80 °C, 1500 psi, 2à 10 min static extractions with flush volume 50%) combined with NPLC system. Typical extractions give 3.7-4.0 mg mlâ1 of Vitamin E isomers and β-carotene of 3.3-3.5 mg mlâ1, which is similar to or greater than classical Soxhlet extraction with n-hexane or chloroform. The new developed method demonstrated an acceptable performance with good efficiency in terms of total extraction time, total solvent usage, total β-carotene and Vitamin E isomers contents as well as exceptional method repeatability.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
M. Marsin Sanagi, H.H. See, Wan Aini Wan Ibrahim, Ahmedy Abu Naim,