Determination of disopyramide in human urine by capillary electrophoresis with electrochemiluminescence detection of tris(2,2′-bipyridyl) ruthenium (II)

Capillary electrophoresis (CE) with electrochemiluminescence detection (ECL) of disopyramide based on tris(2,2′-bipyridyl) ruthenium(II) was described. After CE separation using a 50 μm i.d. uncoated capillary column and 10 mM running buffer solution at 15 kV of separation potential, the analyte was detected at the surface of platinum disk electrode (300 μm in diameter) working electrode. Parameters influencing separation and detection were optimized in detail. Under optimal condition the detection limit was 2.5 × 10−8 mol L−1 (S/N = 3). The method can be successfully applied for the determination of disopyramide in human urine after an easy, rapid and cheap extraction procedure. Extraction recovery rates were between 92.3 and 96.9%.