| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9743876 | Analytica Chimica Acta | 2005 | 7 Pages |
Abstract
The 4-(1â²-cyanoisoindolyl)phenylisothiocyanate (CIPIC) could be used as a chemiluminescence (CL) Edman-type reagent. Firstly, CL reaction conditions such as pH, buffer types, H2O2 concentration and organic solvents were optimized in detail. Secondly, the CL sensitivity of the derivative of Ala was compared with fluorescence and absorbance detections. Thirdly, the structures of the CL intermediate and emitter were elucidated by liquid chromatography-mass spectrometry (LC-MS) and NMR of CIPIC and its analogues after their CL reactions. Consequently, the thiohydantoin derivatives for 16 kinds of amino acids were separated and sensitively detected by CL with lower detection limits of 0.3-0.8Â pmol at a signal-to-noise ratio of 3, after the coupling reaction of amino acids with CIPIC and their cyclisation reaction.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Masaaki Kai, Mikio Morizono, Moses Njoroge Wainaina, Tsutomu Kabashima, Myung Koo Lee, Jianzhong Lu,