Boldenone and related hormones quantification by liquid chromatography-mass spectrometry in urine and faeces after calf administration of boldenone undecanoate

A 6-month calf was treated with 1 mg kg−1 wet weight of 17-β-boldenone undecanoate and urine and faeces were collected for a period of 30 days after the treatment to follow the depletion of the different substances related to boldenone. Hair was collected for just a few days. Multiresidue extraction and purification methods were developed for the different matrices. Separation was carried on a RP-C18 LC column and liquid chromatography-mass spectrometry (LC-MS/MS) detection was performed using an ion trap mass spectrometer equipped with electrospray source for urine and with atmospheric pressure chemical ionisation for faeces. The concentration found for the different analytes was discussed compared to a control animal.