Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9743965 | Analytica Chimica Acta | 2005 | 7 Pages |
Abstract
An effective liquid chromatographic method with tandem mass spectrometric (LC-MS/MS) detection and identification is presented for the determination of chloramphenicol (CAP) in honey. After a preliminary dissolution in water, samples were extracted with a mixture of dichloromethane/acetone and evaporated to dryness; the following clean up was carried out on an octadecyl (C18) SPE cartridge. CAP was determined by LC-MS/MS, using electrospray ionization in the negative ion mode (ESIâ). The column was a LUNA Phenomenex with a mixture of methanol-aqueous ammonium acetate (60:40, v/v) as a mobile phase. Honey samples were fortified at CAP levels 0.30-0.45-0.60 μg kgâ1 with 5D-CAP as internal standard. At these levels, trueness ranged between 98.7 and 102.0% and within-laboratory reproducibility was lower than 6.2%, expressed as relative standard deviation. The limit of decision (CCα) was 0.07 μg kgâ1 and detection capability (CCβ) was 0.10 μg kgâ1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
A.F. Forti, G. Campana, A. Simonella, M. Multari, G. Scortichini,