Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9759297 | International Journal of Hydrogen Energy | 2005 | 9 Pages |
Abstract
Phototrophic hydrogen production was conducted using individual substrates, acetate and butyrate, which are the main products of dark fermentation. Effects of initial pH (ranging 5.0-10.0) and individual substrate concentrations (acetate ranging from 800 to 4100Â mg/l, and butyrate ranging from 1000 to 5100Â mg/l) on phototrophic hydrogen production were evaluated. The maximum hydrogen yields were 2.5Â mol-H2/mol-acetate at an initial pH of 8.0 treating 800Â mg/l of acetate, 3.7Â mol-H2/mol-butyrate at an initial pH of 9.0 treating 1000Â mg/l of butyrate. Analyses of DGGE (denaturing gradient gel electrophoresis) profiles of 16S rDNA fragments and FISH (fluorescent in situ hybridization) images show that both phototrophic hydrogen-producing sludges comprised only one predominant species resembling Rhodobacter capsulatus with over 80% relative abundance.
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Authors
Herbert H.P. Fang, Hong Liu, Tong Zhang,