Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9882359 | Archives of Biochemistry and Biophysics | 2005 | 12 Pages |
Abstract
We have isolated and characterised two neutral oligosaccharides, one nonfucosylated and the other monofucosylated, from human milk that are based on the doubly branched lacto-N-decaose core. Their structures have been determined by a combined use of electrospray tandem mass spectrometry (ES-MS/MS) and NMR spectroscopy. The sequences of the three branches resulted from the double-branching, including the identity and location of the blood-group-related Lewis determinant and partial linkages, were elucidated by the unique method of high sensitivity negative-ion ES-MS/MS analysis. Their full structure assignment was completed by methylation analysis and 1H NMR. The monofucosylated lacto-N-decaose, Galβ1-4(Fucα1-3)GlcNAcβ1-6(Galβ1-3GlcNAcβ1-3)Galβ1-4GlcNAcβ1-6(Galβ1-3GlcNAcβ1-3)Galβ1-4Glc is a novel sequence, whereas the nonfucosylated lacto-N-decaose, Galβ1-4GlcNAcβ1-6(Galβ1-3GlcNAcβ1-3)Galβ1-4GlcNAcβ1-6(Galβ1-3GlcNAcβ1-3)Galβ1-4Glc, has not been isolated and identified as an individual oligosaccharide.
Keywords
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Biochemistry
Authors
Wengang Chai, Vladimir E. Piskarev, Yibing Zhang, Alexander M. Lawson, Heide Kogelberg,