Article ID Journal Published Year Pages File Type
9882398 Archives of Biochemistry and Biophysics 2005 12 Pages PDF
Abstract
This review will focus on established and newly emerging strategies for identifying and characterizing enzyme intermediates using a rapid transient kinetic approach. The merits of this methodology as well as the basics of experimental design are described. Several illustrative examples of PEP-utilizing enzymes have been chosen as they all perform unique, novel chemistries involving enzyme intermediates and have proven to be exciting pharmaceutical targets for antibiotics and herbicides. A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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