Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9885586 | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 2005 | 5 Pages |
Abstract
SCC antigen (SCCA) has been used as a tumor marker for squamous cell carcinoma. Analyses of the SCCA1 and SCCA2 genes, which are almost identical, and their promoters have been reported. Recently it was found that both SCCAs were stimulated by interleukin (IL)-4 and IL-13. Here we analyzed the promoter activity of both SCCAs in the 5â²-flanking region, exon 1, and intron 1 to evaluate a putative STAT6 binding site. The addition of intron 1 to the luciferase assay constructs including the 5â²-flanking region significantly augmented the promoter activity of both SCCA1 and SCCA2. Furthermore, deletion analyses of intron 1 revealed that a 50-bp fragment of intron 1 that includes putative STAT6 binding site was responsible for the increased promoter activity. Although the sequences of SCCA1 and SCCA2 are very similar in the 5â²-flanking region, the analysis of the â337 single nucleotide polymorphism of SCCA2 indicated that this polymorphism may underlie the difference in promoter activity between SCCA1 and SCCA2.
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Biochemistry
Authors
Yoshinori Suminami, Fumio Kishi, Shugo Nawata, Akihiro Murakami, Yuko Sakaguchi, Kotaro Sueoka, Fumitaka Numa, Norihiro Sugino, Hiroshi Kato,