Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9886078 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2005 | 7 Pages |
Abstract
Here, in the experiments of both PCR and real-time PCR, a repression of DNA amplification was observed in the presence of protein tau. Furthermore, a strong repression appeared when an in vitro DNA replication assay was performed at the physiological temperature (37 °C). The incorporation of dNTP was markedly decreased to â¼12% of control by the presence of tau23 and to â¼15% by tau40. In the competitive experiments, the PCR product could be restored when the competitor DNA was added, indicating that the association of tau with the template gave rise to the repression. However, tau did not repress the yield of RNA in transcription, suggesting that tau was replaced or ejected from the template by the elongating T7 RNA polymerase.
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Biochemistry
Authors
Wen Li, Xing Sheng Wang, Mei Hua Qu, Ying Liu, Rong Qiao He,