Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9886098 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2005 | 6 Pages |
Abstract
The glycosaminoglycan hyaluronan (HA) is involved in a variety of functions such as cell migration, adhesion, activation of intracellular signaling, metastasis, inflammation and wound repair. These functions of HA are mediated via HA-binding proteins (HABPs). To derive details of the HA-binding site in HABPs, here, we panned a random peptide display library expressed on the E. coli flagellin protein using HA-coated plates. Using this random peptide display library, 40 positive clones were obtained and the nucleotide sequences were determined. As a result, an Arg-Arg sequence, in addition to the known B-X7-B motif, was found to bind to HA. A binding experiment using the IAsys resonant mirror biosensor verified that a peptide containing an Arg-Arg sequence binds to HA.
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Authors
Kana Amemiya, Tatsuya Nakatani, Akio Saito, Atsuo Suzuki, Hiroshi Munakata,