Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9886162 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2005 | 9 Pages |
Abstract
Food proteins were shown to affect atherogenic risk factors, which is supposed to be related to specific peptide sequences encrypted within their primary sequence. The aim of this study was to evaluate the effects of peptides and hydrolysates from two food proteins, casein and soy protein, on endothelial cell functions (cell proliferation and release of vasoactive substances). Cell proliferation was not influenced by dipeptides and most of the tripeptides, whereas several total hydrolysates from casein and soy protein inhibited cell proliferation at higher concentrations (>0.25 mg/mL; P<0.05). The release of one or more of the vasoactive substances, thromboxan B2 (stable marker of thromboxan A2), 6-keto-prostaglandin F1α (stable marker of prostaglandin I2), endothelin-1, and nitric oxide, was significantly influenced by the incubation with various peptides compared with control cells (P<0.05). Various hydrolysate fractions from casein and soy protein influenced the release of 6-keto-prostaglandin F1α and nitric oxide (P<0.05) but did not influence the release of thromboxan B2 and endothelin-1. In conclusion, the present study demonstrates that peptides and hydrolysate fractions from casein and soy protein influence endothelial cell function as evidenced by the modulation of endothelial cell proliferation and alterations in the release of vasoactive substances.
Keywords
HBSSHEPES buffered salt solutionPGI2TXB2TNSTxA2ET-1ACEmTORTNFα3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide6-keto-PGF1αBSAMTTbovine serum albuminangiotensin-I-converting enzymeEDTAethylenediaminetetraacetateElastaseendothelin-1Trypsintumor necrosis factor-αEndothelial cellVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)Hank's buffered salt solutionNitric oxidemammalian target of rapamycinHydrolysateSoy proteinProstaglandin I2PeptidePepsinCaseinchymotrypsin
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
R. Ringseis, B. Matthes, V. Lehmann, K. Becker, R. Schöps, R. Ulbrich-Hofmann, K. Eder,