Article ID Journal Published Year Pages File Type
9888170 Clinica Chimica Acta 2005 5 Pages PDF
Abstract
315 samples were successfully screened with this new assay. The temperatures in the melting analysis of the SPINK5 exon 14 PCR product were characteristic to the probes hybridised to the mutant (AA) at 51.5 °C and to the wild-type (GG) at 59.5 °C. The fast and reliable mutation detection in the tested samples makes this high-speed method suitable for larger epidemiological studies.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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