Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9890895 | International Journal of Biological Macromolecules | 2005 | 7 Pages |
Abstract
Regiospecific oxidation of the primary hydroxyl groups in lacquer polysaccharide (LPL, Mw 6.85 Ã 104) and its NaIO4 oxidation derivatives (LPLde) to C-6 carboxy groups was achieved with NaOCl in the presence of Tempo® and NaBr. Sulfate groups were incorporated into the oxidated polysaccharides using Py·SO3 complex as a reagent. Reactivity of polysaccharide hydroxyl group was C-6 > C-2 > C-4. Sulfate groups were mainly linked to the second hydroxy at C-2 in the products. The results of APTT assay showed after incorporation of carboxyl groups into lacquer polysaccharides, the intrinsic coagulation pathway was promoted, and all sulfated polysaccharides had very weak anticoagulant activity within the scope of studied DS (0.39-1.11). These indicated that carboxyl groups and sulfate groups had the synergistic action. At the same time, the anticoagulant activity increased very slowly with the DS in the second hydroxy. This indicated that 6-O-SO3â in the side chains took an important role in the anticoagulant activity.
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Authors
Jianhong Yang, Yumin Du, Ronghua Huang, Yunyang Wan, Yan Wen,