| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9890945 | International Journal of Biological Macromolecules | 2005 | 8 Pages |
Abstract
Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Michiko Kaya, Yoshiharu Toyama, Kenji Kubota, Yoshinobu Nodasaka, Masanori Ochiai, Motoyoshi Nomizu, Norio Nishi,