Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9893101 | Microvascular Research | 2005 | 5 Pages |
Abstract
There is a growing body of evidence that the advanced glycation end product (AGE)-their receptor (RAGE) system plays a central role in the pathogenesis of diabetic vascular complication. The renin-angiotensin system (RAS) contributes to the development and progression of diabetic angiopathy as well. However, the cross-talk between the AGE-RAGE system and the RAS is not fully understood. In this study, we examined the role of angiotensin II (Ang II) type 1 receptor system for RAGE expression in cultured endothelial cells (ECs) and in patients with essential hypertension. Ang II up-regulated RAGE mRNA levels of microvascular ECs and subsequently increased the soluble form of RAGE (sRAGE) expression in the medium of ECs, both of which were completely blocked by telmisartan, a commercially available Ang II type 1 receptor antagonist. Furthermore, telmisartan was found to decrease serum levels of sRAGE in patients with essential hypertension. These results demonstrate that sRAGE is released from the cell surface of Ang-II-exposed ECs. Our present study indicates that a cross-talk exists between the AGE-RAGE system and the RAS and suggests that serum levels of sRAGE may reflect endothelial RAGE expression.
Keywords
sRAGERAGEAGEsVCAM-1RASRT-PCRICAM-1receptor for AGEsACENACHDLARBsECshigh-density lipoproteinN-acetylcysteineAngiotensin-converting enzymeAngiotensin IIELISAEnzyme-linked immunosorbent assayAng IIEndothelial cellsRenin–angiotensin systemBlood pressureAdvanced glycation end productsintracellular adhesion molecule-1vascular cell adhesion molecule-1reverse transcription-polymerase chain reactionAT1 receptorANG II type 1 receptor
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Authors
Kazuo Nakamura, Sho-ichi Yamagishi, Yayoi Nakamura, Katsuhiko Takenaka, Takanori Matsui, Yuko Jinnouchi, Tsutomu Imaizumi,