Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9894418 | Regulatory Peptides | 2005 | 8 Pages |
Abstract
Previous studies have shown immunostaining of adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) throughout the gastrointestinal tract. Based on these data, we decided to investigate the effect of these peptides on intestinal sugar absorption using everted rings from Wistar rat intestine. PAMP increases alpha-methylglucoside (MG) uptake at concentrations ranging from 10â12 to 10â7 M. AM shows a dual effect inhibiting sugar absorption at low concentrations (10â12 to 10â11 M) and increasing MG uptake at higher concentrations (10â8 to 10â6 M). In all cases, the effect is phloridzin-sensitive, indicating that the peptides alter SGLT1 function without modifying the non-mediated component of absorption. The enhancing effect of 10â8 M AM and PAMP seems to be mediated by elevation of cAMP and is accompanied by an increase on SGLT1 expression in the brush-border membrane of the enterocytes. The inhibitory effect of 10â12 M AM could be mediated by either cAMP reduction or, more probably, by other second messenger able to inhibit sugar absorption. PKC is not involved in the action of either AM or PAMP. These results demonstrate that both peptides play a role in the regulation of the active transport of sugars in the intestine.
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Authors
I. Fernández de Arcaya, M.P. Lostao, A. MartÃnez, A. Berjón, A. Barber,