Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9918716 | International Journal of Pharmaceutics | 2005 | 5 Pages |
Abstract
This methodology was applied to investigate the intracellular distribution of the nanoparticles after their incubation with the RBEC. The results showed that PEG-PHDCA nanoparticles were able to be internalised to a higher extent than PHDCA nanoparticles (after 20Â min incubation). Additionally, these nanoparticles displayed different patterns of intracellular capture, depending on their specific surface composition: PEG-PHDCA nanoparticles were 48% in the plasma membrane, 24% in the cytoplasm, 20% in vesicular compartments and 8% associated with the fraction of the nucleus, the cytoskeleton and caveolae suggesting that PEG-PHDCA nanoparticle uptake by RBEC is specific and presumably due to endocytosis. Confocal microscopy studies confirmed the cellular uptake of PEG-PHDCA nanoparticles.
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Authors
Elizabeth Garcia-Garcia, Karine Andrieux, Sophie Gil, Hyun Ryoung Kim, Trung Le Doan, Didier Desmaële, Jean d'Angelo, Frédéric Taran, Dominique Georgin, Patrick Couvreur,