Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9954155 | The International Journal of Biochemistry & Cell Biology | 2018 | 25 Pages |
Abstract
The light emitting diodes (LEDs) irradiation has been demonstrated to be potential therapeutic strategies for several diseases. However, the blue LED effects remain largely unknown in colorectal cancer (CRC), which is a major cause of morbidity and mortality throughout the world. In this study, we determined the effects of blue LED irradiation, the maximal light emission at 470ânm in wavelength, in human CRC cell lines SW620 and HT29. The cells were irradiated with blue LED light for 0âJ/cm2, 72âJ/cm2, 144âJ/cm2, 216âJ/cm2 and 288âJ/cm2 respectively. We found that irradiation with blue LED light induced a marked decrease of live cells and an increase of dead cells. Additionally, lower cell proliferation and a remarkably increase of cell apoptosis were observed in blue LED-irradiated cells as compared with non-irradiated control group. The cell migration was significantly inhibited by blue LED irradiation 24, 48 and 72âh later compared with non-treated group. Blue LED-treated CRC cells further displayed a remarkably inhibition of EMT process in CRC cells. Finally, we found the accumulation of ROS production and DNA damage were induced by blue LED irradiation. These results indicated that blue LED irradiation inhibits CRC cell proliferation, migration and EMT process as well as induces cell apoptosis, which may result from increased ROS accumulation and induction of DNA damage.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Gege Yan, Lai Zhang, Chao Feng, Rui Gong, Elina Idiiatullina, Qihe Huang, Mingyu He, Shuyuan Guo, Fan Yang, Yuan Li, Fengzhi Ding, Wenya Ma, Valentin Pavlov, Zhenbo Han, Zhiguo Wang, Chaoqian Xu, Benzhi Cai, Ye Yuan, Lei Yang,