Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10137372 | Research in Veterinary Science | 2018 | 8 Pages |
Abstract
This study evaluated the effects of frutalin (0.6, 6.0 or 60.0â¯Î¼g/mL) and doxorubicin (0.3â¯Î¼g/mL) on survival, growth and ultrastructure of in-vitro cultured goat secondary follicles. The effects of these substances on the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 were also investigated. Results showed that, after 6â¯days of culture, frutalin or doxorubicin reduced the percentage of normal follicles (Pâ¯<â¯0.05), but doxorubicin had higher toxicity than frutalin. Except for follicles cultured with 0.6â¯Î¼g/mL frutalin, follicular growth rate was reduced after culture with doxorubicin or frutalin (Pâ¯<â¯0.05). The presence doxorubicin or 60.0â¯Î¼g/mL frutalin increased the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 (Pâ¯<â¯0.05). Higher mRNA levels for Casp3, Casp6 and Bax were found in follicles cultured with doxorubicin, but higher levels of Bcl2 mRNA were found in follicles cultured with frutalin (Pâ¯<â¯0.05). In conclusion, frutalin has lower toxic effects than doxorubicin on secondary follicles cultured in vitro.
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Authors
M.A.A. Soares-Costa, J.J.N. Costa, A.W.B. Silva, E.V. Cunha, L.R.F.M. Paulino, B.R. Silva, A.L.C. Silva, R. van den Hurk, J.R.V. Silva,