Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10138793 | Aquatic Toxicology | 2018 | 9 Pages |
Abstract
This study examined the impacts of Macondo oil from the Deepwater Horizon oil spill, both alone and in conjunction with exposure to the known fish pathogen Vibrio anguillarum, on the expression of five immune-related gene transcripts of red snapper (il8, il10, tnfa, il1b, and igm). In order to elucidate this impact, six different test conditions were used: one Control group (No oil/No pathogen), one Low oil/No pathogen group (tPAH50â=â0.563âμg/L), one High oil/No pathogen group (tPAH50â=â17.084âμg/L, one No oil/Pathogen group, one Low oil/Pathogen group (tPAH50â=â0.736âμg/L), and one High oil/Pathogen group (tPAH50â=â15.799âμg/L). Fish were exposed to their respective oil concentrations for one week. On day 7 of the experiment, all fish were placed into new tanks (with or without V. anguillarum) for one hour. At three time points (day 8, day 10, and day 17), fish organs were harvested and placed into RNAlater, and qPCR was run for examination of the above specific immune genes as well as cyp1a1. Our results suggest that cyp1a1 transcripts were upregulated in oil-exposed groups throughout the experiment, confirming oil exposure, and that all five immune gene transcripts were upregulated on day 8, but were generally downregulated or showed no differences from controls on days 10 and 17. Finally, both oil and pathogen exposure had impacts on growth.
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Aquatic Science
Authors
Maria L. Rodgers, Ryan Takeshita, Robert J. Griffitt,