| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10142974 | Plant Physiology and Biochemistry | 2018 | 7 Pages |
Abstract
To examine intracellular localization of PtIGS, crude leaf extracts were separated into cytosol and microsome fractions, and found PtIGS in the cytosol and in microsome fractions. Furthermore, microsomal PtIGS was soluble in the presence of detergents and urea and was strongly associated with membranes. Finally, we confirmed endoplasmic reticulum (ER) membrane localization of PtIGS using ultracentrifugation with a sucrose density gradient. These data suggest that PtIGS interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate.
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Plant Science
Authors
Shintaro Inoue, Rihito Morita, Keiko Kuwata, Tadashi Kunieda, Haruko Ueda, Ikuko Hara-Nishimura, Yoshiko Minami,