Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10149726 | Industrial Crops and Products | 2018 | 9 Pages |
Abstract
This study attempts to develop a reproducible protocol for in vitro adventitious root regeneration for enhanced biomass production from nodal segments of Allamanda cathartica L., which may be of use as an alternative source for production of iridoid glycosides. Allamanda cathartica (Apocynaceae) is a medicinally rich vine, used in the treatment of human carcinoma, human immunodeficiency virus (HIV), jaundice and malaria. The roots of the plant are the main reservoir of secondary metabolites. The main active constituent in root is iridoid glycosides. The explant for root initiation was cultured in ½ strength Murashige and Skoog (MS) liquid medium augmented with different levels of Indole-3-butyric acid. 0.5âμM Indole-3-butyric acid with 4% sucrose resulted in 1.810â±â0.049 g and 0.376â±â0.053âg fresh and dry weight respectively. Among different levels of sodium chloride (80, 100, 120 and 150âmM) added to optimized media, 120âmM sodium chloride treatment increased the root biomass considerably compared with Indole-3-butyric acid alone and other combination, and recorded the fresh weight 2.090â±â0.049âg and dry weight 0.400â±â0.005âg after 12 weeks of incubation. The phytochemical investigations using high performance thin-layer liquid chromatography (HPTLC) and mass spectrometry (MS) analysis revealed concentration dependent modulation of selected iridoids in the cultures treated with sodium chloride. The protocol developed can be utilized as an alternative source for production of iridoid glycosides and the findings may be explored on industrial scale.
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Authors
Mehrun Nisha Khanam, Mohammad Anis, Sayeed Ahmad,