Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10158048 | Journal of Dairy Science | 2018 | 11 Pages |
Abstract
Pathogens, mycotoxins, or antibiotics may exist in a food sample. Micro- and macromolecular substances must be detected quickly. A rapid and convenient lateral flow immunoassay (LFI) integrated with competitive and sandwich models was developed to detect micro- and macromolecular substances. In this study, aflatoxin M1 (AFM1) and Escherichia coli O157:H7 were selected as the micro- and macromolecular substances, respectively. Two test lines in the LFI test strip were evaluated to detect AFM1 and E. coli O157:H7 by competitive and sandwich models. Results showed that the limits of detection for detecting AFM1 and E. coli O157:H7 were 50 pg·mLâ1 and 1.58 à 104 cfu·mLâ1, respectively. The whole assay time was 30 min. The recoveries of gold nanoparticle-LFI ranged from 78.0 to 111.6% with coefficients of variation in the range of 3.9 to 8.5% for the detection of AFM1. For the detection of E. coli O157:H7, the range of recoveries was from 70.1 to 89.6% with coefficients of variation ranging from 4.9 to 13.0%. This study not only tested sensitivity and specificity, but also was a systematic study of location of 2 test lines of the LFI test strip integrated with competitive and sandwich models.
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Authors
Chun Wang, Juan Peng, Dao-Feng Liu, Ke-Yu Xing, Gang-Gang Zhang, Zhen Huang, Song Cheng, Fang-Fei Zhu, Miao-Lin Duan, Kai-Yi Zhang, Mei-Fang Yuan, Wei-Hua Lai,