Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10261112 | Chemical Engineering Journal | 2005 | 8 Pages |
Abstract
The optimal conditions for the growth of a diatom Chaetoceros calcitrans were investigated in a 2.5 L glass bubble column. The light intensity for the highest growth rate was shown to be at around 400 μmol photons mâ2 sâ1. A modified standard F/2 medium with a two-fold of silica and phosphorus concentrations was illustrated to result in a better growth of this diatom. Vitamin B12 in the range from 1 to 3 μg Lâ1 did not significantly affect the growth. A maximum cell density obtained from this small glass bubble column was approximately 5.8 Ã 106 cells mLâ1 with a maximum specific growth rate of 3.80 Ã 10â2 hâ1. The cultivation of C.calcitrans in the 17 L airlift photobioreactor (ALPBR) was illustrated to be superior to that in the bubble column with the same size. The operation with superficial gas velocity of 3 cm sâ1 was found to give a maximum specific growth rate of 7.41 Ã 10â2 hâ1 with a maximum cell concentration of 8.88 Ã 106 cells mLâ1 in a batch culture. A semi-continuous culture could be achieved where the harvest was performed at every 12 h. In this case, the maximum specific growth rate (μ) achievable was 9.65 Ã 10â2 hâ1 and the cell concentration during the harvest period was 4.08 Ã 106 cells mLâ1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Sontaya Krichnavaruk, Worapannee Loataweesup, Sorawit Powtongsook, Prasert Pavasant,