Optimisation des solutions de lavage pour déplasmatiser des concentrés de plaquettes

Different washing and homogénéisation solutions are hereby analysed by comparing the evolution of fonctional indicators during the preservation of washed aphaeresis platelet concentrate: physiological pH 4.5 and 6 solutions, battered physiological pH 6.8 glucose solution, and two physiological pH 7 citrate solutions with acetate. Prior acidification of platelet concentrates proved to be essential. Two whashings with manual or automated technique, guarantee residual proteins at a level of less than 0.5 g. Solutions T-Sol Baxter or SSP Macopharma allow us to obtain a product that meet the PSL spécifications. Routine since June 2004, washings are down with a physiological pH 6 solution, then homogeneised with T-Sol solution. Platelet recovery, swirling phenomenon, lack of agrgegates, pH maintenance, low increase in the platelet average volume and maintenance of intra-cell potassium level, suggest that platelet entirety is preserved beyond the product's expiry date. The platelet transfusion yield of these products is satisfactory.