Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10591498 | Bioorganic & Medicinal Chemistry Letters | 2013 | 5 Pages |
Abstract
Short interfering RNA (siRNA) has been proven to be an utilizable tool for post-transcriptional gene silencing research. In this study, we designed and synthesized two glucosamine analogues and tried to modify the siRNA using these two glucosamine analogues at the 3â²-overhang region of siRNAs to improve the nuclease resistance and to overcome some other weak points. The siRNAs modified with glucosamine analogues had almost no effect of the thermal stability and showed strong resistance to nuclease degradation. Some of them kept the same gene silencing activity level as unmodified siRNA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Xiong Luo, Takahiro Sugiura, Remi Nakashima, Yoshiaki Kitamura, Yukio Kitade,