Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10591595 | Bioorganic & Medicinal Chemistry Letters | 2014 | 4 Pages |
Abstract
High-quality wheat germ extract (hqWGE) is very useful for the high-yield production of various types of protein. The most important key to high productivity is the design of mRNA templates. Although the design has been refined for straightforward and efficient translation in hqWGE, there is still room for improvement in untranslated regions (UTRs), especially the 3â² UTR length, because a long, cumbersome 3â² UTR is commonly used for translation enhancement. Here we examined some short viral 3â² cap-independent translation enhancers (3â² CITEs) to identify effective ones for efficient translation in hqWGE. We then combined the most effective 3â² CITE and a 5â² enhancer to further increase the translation efficiency. mRNA with the optimal short 3â² and 5â² UTRs, both of whose length was less than 150Â nt, exhibited a productivity of 1.4Â mg/mL in prolonged large-scale protein synthesis in hqWGE, which was comparable to that of control mRNA with a commonly-used long 3â² UTR (â¼1200Â nt).
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Atsushi Ogawa, Junichiro Tabuchi, Yasunori Doi,