Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10591768 | Bioorganic & Medicinal Chemistry Letters | 2014 | 4 Pages |
Abstract
Several triplex-forming oligonucleotides (TFOs) partially modified with 2â²-O-(2-aminoethyl)- or 2â²-O-(2-guanidinoethyl)-nucleotides were synthesized and their association rate constants (kon) with double-stranded DNA were estimated by UV spectrophotometry. Introduction of cationic modifications in the 5â²-region of the TFOs significantly increased the kon values compared to that of natural TFO, while no enhancement in the rate of triplex DNA formation was observed when the modifications were in the middle and at the 3â²-region. The kon value of a TFO with three adjacent cationic modifications at the 5â²-region was found to be 3.4 times larger than that of a natural one. These results provide useful information for overcoming the inherent sluggishness of triplex DNA formation.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Yoshiyuki Hari, Shin Ijitsu, Masaaki Akabane-Nakata, Takuya Yoshida, Satoshi Obika,