Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10592476 | Bioorganic & Medicinal Chemistry Letters | 2012 | 4 Pages |
Abstract
Fluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2â²-deoxyguanosine were synthesized and converted into the corresponding 5â²-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2â²-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Bastian Holzberger, Julian Strohmeier, Vanessa Siegmund, Ulf Diederichsen, Andreas Marx,