Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10594386 | Bioorganic & Medicinal Chemistry Letters | 2012 | 6 Pages |
Abstract
This Letter describes a method for enriching C-terminal peptide of protein for C-terminal sequence analysis. This method employs endopeptidase ArgC digestion and C-terminal peptide enrichment using m-aminophenylboronic acid-agarose as an arginine-capture material. The selectively recovered C-terminal peptide incorporates no artificial derivatization. Therefore, the widely used functional groups (e.g. α-NH2 and α-COOH) can be used for any necessary transformation. In this research, a TMPP mass tag was attached to the α-NH2 group to clarify the amino acid sequence of the C-terminal peptide.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Hiroki Kuyama, Chihiro Nakajima, Koichi Tanaka,