Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10594511 | Bioorganic & Medicinal Chemistry Letters | 2012 | 4 Pages |
Abstract
Chemically modified short interfering RNAs (siRNAs) that contain backbone amide-bonds at both 3â²-overhangs and internal positions were synthesized. These siRNAs contain the modifications within both the sense and antisense strands that target the transcripts from pGL2 and pGL3. The siRNAs were synthesized via site-specific incorporation of a PNA-RNA dimer by solid-phase phosphoramidite techniques. The silencing data suggest a high degree of compatibility of amide-modified siRNAs within the RNAi pathway when located internally within the sense strand and at 3â²-overhangs. Biophysical data indicates that melting temperatures of the siRNAs decrease when the modification is located internally.
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Authors
Wei Gong, Jean-Paul Desaulniers,