| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10595732 | Bioorganic & Medicinal Chemistry Letters | 2011 | 6 Pages |
Abstract
We have developed an approach that allows relative quantification of protein sulfenic acids using a pair of light and heavy isotope labled probes, DAz-2 and d6-DAz-2. In conjunction with a new complementary acid-cleavable linker, Yn-ACL, we demonstrate that tagged peptides are successfully labeled, enriched, and fully characterized by LC-MS/MS analysis. Overall, this method can be applied to map sites of cysteine oxidation and compare protein sulfenylation in normal and disease states.
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Authors
Thu H. Truong, Francisco J. Garcia, Young Ho Seo, Kate S. Carroll,