Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10595737 | Bioorganic & Medicinal Chemistry Letters | 2011 | 4 Pages |
Abstract
Metabolic oligosaccharide engineering is a powerful approach for installing unnatural glycans with unique functional groups into the glycocalyx of living cells and animals. Using this approach, we showed that K+ channel complexes decorated with thiol-containing sialic acids were irreversibly inhibited with scorpion toxins bearing a pendant maleimide group. Irreversible inhibition required a glycosylated K+ channel subunit and was completely reversible with mild reductant when the tether connecting the toxin to the maleimide contained a disulfide bond. Cleavage of the disulfide bond not only restored function, but delivered a biotin moiety to the modified K+ channel subunit, providing a novel approach for preferentially labeling wild type K+ channel complexes functioning in cells.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Zhengmao Hua, Anatoli Lvov, Trevor J. Morin, William R. Kobertz,