Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10595935 | Bioorganic & Medicinal Chemistry Letters | 2013 | 8 Pages |
Abstract
We have developed a methodology for quantitative analysis and concurrent identification of proteins by the modification of cysteine residues with a combination of iodoacetanilide (IAA, 1) and 13C7-labeled iodoacetanilide (13C7-IAA, 2), or N-ethylmaleimide (NEM, 3) and d5-labeled N-ethylmaleimide (d5-NEM, 4), followed by mass spectrometric analysis using nano liquid chromatography/nanoelectrospray ionization ion trap mass spectrometry (nano LC/nano-ESI-IT-MS). The combinations of these stable isotope-labeled and unlabeled modifiers coupled with LC separation and ESI mass spectrometric analysis allow accurate quantitative analysis and identification of proteins, and therefore are expected to be a useful tool for proteomics research.
Related Topics
Physical Sciences and Engineering
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Organic Chemistry
Authors
Sadamu Kurono, Yuka Kaneko, Satomi Niwayama,