| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10595949 | Bioorganic & Medicinal Chemistry Letters | 2013 | 4 Pages |
Abstract
We designed a new alkaline phosphatase (ALP)-sensitive fluorogenic probe in which a self-immolative spacer group, p-hydroxybenzyl alcohol, is linked to a profluorogenic compound to improve substrate specificity. Enzymatic hydrolysis converts the fluorogenic substrate 1 to a highly fluorescent reporter 3, thus allowing for the fast and quantitative analysis of ALP activity with greatly increased affinity for the enzyme.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jeesook Park, Youngmi Kim,