Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10596632 | Bioorganic & Medicinal Chemistry Letters | 2012 | 4 Pages |
Abstract
Chemical investigations on the EtOAc-soluble fractions from the EtOH extract of Formosa soft coral afforded a new 9,11-secosteroid, 8αH-3β,11-dihydroxy-5α,6α-expoxy-24-methylene-9,11-secocholestan-9-one (1), along with one known steroid 3β,11-dihydroxy-5β,6β-expoxy-24-methylene-9,11-secocholestan-9-one (2) from Sinularia granosa. The structure of the new metabolite was elucidated on the basis of extensive spectroscopic analysis and by comparison of their NMR data with the known compounds, including 2. Both 1 and 2 were shown to significantly inhibit the accumulation of the pro-inflammatory inducible nitric oxide synthase protein, and 1 also was found to effectively reduce the level of cyclooxygenase-2 protein, in lipopolysaccharide-stimulated RAW264.7 macrophage cells at 10 μM. Furthermore, cytotoxic activity of 1 and 2 toward a limited panel of cancer cell lines was also discovered.
Keywords
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Chiung-Yao Huang, Jui-Hsin Su, Chang-Yih Duh, Bo-Wei Chen, Zhi-Hong Wen, Yao-Haur Kuo, Jyh-Horng Sheu,