| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10598331 | Bioorganic & Medicinal Chemistry Letters | 2005 | 5 Pages |
Abstract
A unique chromism-based assay (CHROBA) technique using photochromic spiropyran-containing peptides has been firstly established for detection of protein kinase A-catalyzed phosphorylation. The promising method has advantages that avoid isolation and/or immobilization of substrates to remove excess nonreactive isotope-labeled ATP or fluorescently-labeled anti-phosphoamino acid antibodies from the reaction mixture.
Related Topics
Physical Sciences and Engineering
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Authors
Kin-ya Tomizaki, Xu Jie, Hisakazu Mihara,