Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10598832 | Bioorganic & Medicinal Chemistry Letters | 2005 | 4 Pages |
Abstract
Procedures for synthesizing α-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CLpro) as a model for attachment to chips containing d-/l-Glu, Asp, Orn, Ser via different transitional metal ions, it was found that the Orn chip was the best of affinity binding and stability by which Zn2+ was the best metal ion for affinity while Co2+ was the best metal ion for stability. Thus, this protein microarray can be alternatively used as a high throughput screening method for rapid detection against SARS CoV 3CLpro and/or efficient purification of other Tagged proteins.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Supachok Sinchaikul, Fu-Ming Pan, Ching-Wen Cheng, Chi-Huey Wong, Jim-Min Fang, Min-Jen Tseng, Shui-Tein Chen,