Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10795806 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | 2013 | 30 Pages |
Abstract
⺠Eleven single-reactive cysteine variants of cytochrome c oxidase (CcO) from P. denitrificans were constructed. ⺠Modification of the cysteine residues with the fluorescent dye fluorescein was demonstrated. ⺠A distinct polarity pattern for the fluorescein attachment sites at the entrance of the D- and K-channel of CcO was observed. ⺠The cytoplasmic surface of CcO becomes more apolar upon reduction of the enzyme.
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Authors
Kristina Kirchberg, Hartmut Michel, Ulrike Alexiev,