Physiology and global gene expression of a Corynebacterium glutamicum ΔF1FO-ATP synthase mutant devoid of oxidative phosphorylation

► We describe a C. glutamicum mutant with a deletion of the F1FO-ATP synthase genes. ► Growth of DF1FO mutant proves that oxidative phosphorylation is dispensable. ► Under O2 excess DF1FO shows increased glucose consumption, respiration and DΨ. ► Transcriptomics reveals ~ 300 genes with ≥2-fold altered mRNA levels in DF1FO. ► Proteomics reveals ~ 30 proteins with ≥1.5-fold altered protein levels in in DF1FO.