Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10795966 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | 2010 | 8 Pages |
Abstract
Besides triiodothyronine (T3), 3,5-diiodo-L-thyronine (T2) has been reported to affect mitochondrial bioenergetic parameters. T2 effects have been considered as independent of protein synthesis. Here, we investigated the effect of in vivo chronic T2 administration to hypothyroid rats on liver mitochondrial FoF1-ATP synthase activity and expression. T2 increased state 4 and state 3 oxygen consumption and raised ATP synthesis and hydrolysis, which were reduced in hypothyroid rats. Immunoblotting analysis showed that T2 up-regulated the expression of several subunits (α, β, FoI-PVP and OSCP) of the ATP synthase. The observed increase of β-subunit mRNA accumulation suggested a T2-mediated nuclear effect. Then, the molecular basis underlying T2 effects was investigated. Our results support the notion that the β-subunit of ATP synthase is indirectly regulated by T2 through, at least in part, the activation of the transcription factor GA-binding protein/nuclear respiratory factor-2. These findings provide new insights into the T2 role on bioenergetic mechanisms.
Keywords
RPA6-n-propyl-2-thiouracilNuclear respiratory factor-2eSMPFoF1-ATP synthasePTUOSCPRCIfT3FT4iOp3,5-diiodo-l-thyronine3,5-DiiodothyronineBSATHsRNAse protection assaybovine serum albuminiopanoic acidchromatin immunoprecipitationanalyses of varianceANOVAFree thyroxineRespiratory control indexOxidative phosphorylationGrowth hormoneThyroid hormonesCHiP
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Authors
Roberto Mangiullo, Antonio Gnoni, Fabrizio Damiano, Luisa Siculella, Franco Zanotti, Sergio Papa, Gabriele V. Gnoni,