Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10796040 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | 2008 | 6 Pages |
Abstract
Aerobically grown yeast cells express mitochondrial lactate dehydrogenases that localize to the mitochondrial inner membrane. The d-lactate dehydrogenase is a zinc-flavoprotein with high acceptor specificity for cytochrome c, that catalyzes the oxidation of d-lactate into pyruvate. In this paper, we show that mitochondrial respiratory rate in phosphorylating or non-phosphorylating conditions with d-lactate as substrate is stimulated by carboxylic acids. This stimulation does not affect the yield of oxidative phosphorylation. Furthermore, this stimulation lies at the level of the d-lactate dehydrogenase. It is non-competitive, hyperbolic and its dimension is directly related to the number of carboxylic groups on the activator. The physiological meaning of such a regulation is discussed.
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Authors
Arnaud Mourier, Julie Vallortigara, Edgar D. Yoboue, Michel Rigoulet, Anne Devin,