Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10796051 | Biochimica et Biophysica Acta (BBA) - Bioenergetics | 2008 | 6 Pages |
Abstract
Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0Â ng/million cells (7.9Â ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20Â mM glucose, and varied from 1.0-4.4Â ng/million cells (2.7-14.5Â ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1Â h.
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Authors
Vian Azzu, Charles Affourtit, Eamon P. Breen, Nadeene Parker, Martin D. Brand,